TFF1 anticorps (AA 22-87)

N° du produit ABIN5518960

L’anticorps Lapin Polyclonal anti-TFF1 a été validé pour WB, IHC et ELISA. Il convient pour détecter TFF1 dans des échantillons de Souris.

Aperçu rapide pour TFF1 anticorps (AA 22-87) (ABIN5518960)

Antigène: TFF1 (Trefoil Factor 1 (TFF1))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TFF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-TFF1 anticorps

Épitope: AA 22-87

Fonction: Anti-Estrogen Inducible Protein pS2/Tff1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Estrogen Inducible Protein pS2/Tff1 Antibody Picoband® (ABIN5518960). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived mouse Tff1 recombinant protein (Position: Q22-F87). Mouse Tff1 shares 67.2% and 83.6% amino acid (aa) sequence identity with human and rat Tff1, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse
ELISA(Cap), 1-5 μg/mL, Mouse
1. Chutipongtanate, S., Nakagawa, Y., Sritippayawan, S., Pittayamateekul, J., Parichatikanond, P., Westley, B. R., May, F. E. B., Malasit, P., Thongboonkerd, V.Identification of human urinary trefoil factor 1 as a novel calcium oxalate crystal growth inhibitor.J. Clin. Invest. 115: 3613-3622, 2005. 2. Hanby, A. M., Poulsom, R., Singh, S., Elia, G., Jeffery, R. E., Wright, N. A.Spasmolytic polypeptide is a major antral peptide: distribution of the trefoil peptides human spasmolytic polypeptide and pS2 in the stomach.Gastroenterology 105: 1110-1116, 1993. 3. Roberts, M., Wallace, J., Jeltsch, J.-M., Berry, M.The 5-prime flanking region of the human pS2 gene mediates its transcriptional activation by estrogen in MCF-7 cells.Biochem. Biophys. Res. Commun. 151: 306-313, 1988.

Commentaires:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur TFF1

Antigène: TFF1 (Trefoil Factor 1 (TFF1))

Autre désignation: Tff1

Sujet:

Synonyms: Trefoil factor 1,Protein pS2,Tff1,Bcei, Ps2,

Tissue Specificity: Strong expression found in normal gastric mucosa. Not detected in the small or large intestine nor in normal mammary gland.

Background: TFF1 (Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera ed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

Poids moléculaire: 17 kDa

ID gène: 21784

UniProt: Q08423

Pathways: EGFR Signaling Pathway