NSG1 anticorps (N-Term)

N° du produit ABIN5539461

L’anticorps Chèvre Polyclonal anti-NSG1 a été validé pour WB, ELISA et ICC. Il convient pour détecter NSG1 dans des échantillons de Souris et Rat. Il y a 1 publication disponible.

Aperçu rapide pour NSG1 anticorps (N-Term) (ABIN5539461)

Antigène: NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))

Reactivité: Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NSG1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Classe de qualité: Verified

Détail du produit anti-NSG1 anticorps

Épitope: N-Term

Fonction: Nsg1 / Neep21

Séquence: EKGTKQPLLE DGFD

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 22 kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9 kDa according to NP_035072.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of EB11637 (red) in the neurons only. Recommended concentrat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-NSG1 (ABIN5539461)

Digilio, Yap, Winckler et al.: "Ctip2-, Satb2-, Prox1-, and GAD65-Expressing Neurons in Rat Cultures: Preponderance of Single- and Double-Positive Cells, and Cell Type-Specific Expression of Neuron-Specific Gene Family Members, ..." dans: PLoS ONE, Vol. 10, Issue 10, pp. e0140010, (2015) (PubMed).

Détails sur NSG1

Antigène: NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))

Autre désignation: Nsg1

Sujet: Nsg1, neuron specific gene family member 1, Bsmrb, Neep21, PEP1, brain neuron cytoplasmic protein 1/2, brain specific mRNA b, neuron-specific protein family member 1

ID gène: 27065, 18196, 25247

NCBI Accession: NP_077042