CRTC2 anticorps (C-Term)

N° du produit ABIN5539499

Cet anticorps Chèvre Polyclonal détecte spécifiquement CRTC2 dans WB, ELISA, IF et FACS. Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour CRTC2 anticorps (C-Term) (ABIN5539499)

Antigène: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CRTC2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-CRTC2 anticorps

Épitope: C-Term

Fonction: CRTC2

Séquence: DPAVEDSFRS DRLQ

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 90 kDa observed in lysates of cell line Jurkat and in preliminary testing of NIH3T3, and approx 85 kDa in Rat Brain lysates and lysates of cell line A431 (calculated MW of 73.2 kDa according to Human, NP_083157.1) and Rat NP_001029067

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur CRTC2

Antigène: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Autre désignation: CRTC2

Sujet: Crtc2, CREB regulated transcription coactivator 2, 4632407F12Rik, Torc2, OTTMUSP00000023505, transducer of regulated cAMP response element-binding protein (CREB) 2

ID gène: 200186, 74343, 310615

NCBI Accession: NP_083157

Pathways: AMPK Signaling, Carbohydrate Homeostasis