NSG2 anticorps (C-Term)

N° du produit ABIN5539930

Cet anticorps anti-NSG2 est un anticorps Chèvre Polyclonal détectant NSG2 dans ELISA, WB et ICC. Adapté pour Souris et Rat. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour NSG2 anticorps (C-Term) (ABIN5539930)

Antigène: NSG2 (HMP19) (Neuron-Specific Protein Family Member 2 (HMP19))

Reactivité: Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NSG2 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC)

Classe de qualité: Verified

Détail du produit anti-NSG2 anticorps

Épitope: C-Term

Fonction: Nsg2 (mouse, C terminus)

Séquence: HEPKPPKTQG H

Specificité: Reported variants represent identical protein: NP_001277610.1, NP_001277609.1, NP_032767.1

Réactivité croisée: Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 19 kDa band observed in Mouse fetal Brain lysates (calculated MW of 19.0 kDa according to NP_032767.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Rat: Chan

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunocytochemistry: This antibody has been successfully used in ICC on Rat: Chan Choo Yap et al. (2022) PMID: 35474277.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-NSG2 (ABIN5539930)

Yap, Digilio, McMahon, Garcia, Winckler: "Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes." dans: The Journal of cell biology, Vol. 217, Issue 9, pp. 3141-3159, (2019) (PubMed).

Yap, Digilio, McMahon, Winckler: "The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes." dans: Scientific reports, Vol. 7, Issue 1, pp. 10481, (2019) (PubMed).

Détails sur NSG2

Antigène: NSG2 (HMP19) (Neuron-Specific Protein Family Member 2 (HMP19))

Autre désignation: Nsg2

Sujet: Nsg2, neuron specific gene family member 2, protein 8.5, protein p19

ID gène: 18197

NCBI Accession: NP_032767