Cet anticorps anti-Intestinal Alkaline Phosphatase est un anticorps Lapin Polyclonal détectant Intestinal Alkaline Phosphatase dans WB et EIA. Adapté pour Boeuf (Vache).
N° du produit ABIN5539993
Aperçu rapide pour Intestinal Alkaline Phosphatase anticorps (Biotin) (ABIN5539993)
This product detects bovine Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Alkaline phosphatase [calf intestine].
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Western Blot : 1/500-1/2,000. ELISA : 1/4,000-1/20,000. This antibody has been assayed against 1.0 μg of Alkaline phosphatase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
Restrictions
For Research Use only
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.