GAP43 anticorps (C-Term)

N° du produit ABIN5540033

Cet anticorps Poulet Polyclonal détecte spécifiquement GAP43 dans WB, IF, ICC et IHC (p). Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour GAP43 anticorps (C-Term) (ABIN5540033)

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivité: Souris

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp GAP43 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GAP43 anticorps

Épitope: C-Term

Specificité: Specific for the ~43k GAP-43 protein.

Purification: Affinity Chromatography

Immunogène: Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of Mouse GAP-43 coupled to keyhole limpet hemocyanin. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1/10 with PBS containing BSA as a carrier. Finally, the antibody preparation was filter-sterilized.

Isotype: Ig Fraction

Information d'application

Indications d'application: Quality Control: Quality assurance analysis was performed using Immunohistochemistry (at 1/1000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/1000 dilution) as a secondary antibody. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemstry and Immunocytochemistry using 2 % paraformaldehyde-fixed tissues or cells and 1/2000-1/5000 for western blots.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH 7.2 Preservatives: 0.02 % Sodium Azide Stabilizers: 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 2-8°C. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur GAP43

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Autre désignation: gap43

Sujet: GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

UniProt: P17677