CD51 anticorps

N° du produit ABIN5540182

Cet anticorps anti-CD51 est un anticorps Souris Monoclonal détectant CD51 dans FACS, IP, IHC (fro) et EIA. Adapté pour Humain.

Aperçu rapide pour CD51 anticorps (ABIN5540182)

Antigène: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD51 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: NKI-M7 (former AMF7)

Détail du produit anti-CD51 anticorps

Specificité: T-lymphocyte clones NKI-M7 detect a melanoma-associated proteoglycan (MW greater than 450-250 kDa) and a molecular complex, which under reducing conditions consists of 4 compounds of 120, 95, 29 and 25 kDa respectively. reacts with cultured melanomas melanomas in frozen sections. The antigen detected by is strongly associated with the adhesion and cytoplasmic spreading of melanoma cells to plastic surfaces and monolayers of vascular endothelial cells. blocks Fibronectin-induced chemotaxic motility and chemokinesis of melanoma cells. In addition to their membrane localization, the antigens detected by NKI-M7 are abundant in extracellular adhesion plaques deposited by cultured melanoma cells. stains primary tumors heterogeneously, whereas metastases are homogeneously stained.

Purification: Purified

Immunogène: Derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cultured melanoma cells derived from a Human melanoma metastasis.

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Fow Cytometry. Immunoprecipitation. Immunohistochemistry on Frozen Sections.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CD51

Antigène: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Autre désignation: cd51,itgav

Sujet: CD51 (Cluster of Differentiation 51), known as vitronectin receptor α chain, or integrin αV is a type I integral membrane glycoprotein. CD51 forms a heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling, mediates bone absorption and angiogenesis. CD51 is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

UniProt: P06756

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex