Small Basement Membrane Heparan Sulfate Proteoglycan anticorps

N° du produit ABIN5540680

Cet anticorps anti-Small Basement Membrane Heparan Sulfate Proteoglycan Monoclonal Souris (Clone 1F10-B8) (ABIN5540680) détecte spécifiquement Small Basement Membrane Heparan Sulfate Proteoglycan dans WB, EIA et IHC (fro). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Small Basement Membrane Heparan Sulfate Proteoglycan anticorps (ABIN5540680)

Antigène: Small Basement Membrane Heparan Sulfate Proteoglycan

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1F10-B8

Détail du produit

Specificité: This Monoclonal antibody 1F10/B8 is specific for a core protein epitope of a Human small basement membrane Heparan Sulfate proteo-glycan (HS-PG). AM02102PU recognizes an epitope different from that recognized by clones 2H7/G11 (Cat.-No AM02103PU) and 2E2/B5 (Cat.-No AM20231PU). Species: Human.

Purification: Protein G Chromatography

Immunogène: Purified Human small basement membrane Heparan Sulfate Proteoglycan (HS-PG)

Isotype: IgG1

Information d'application

Indications d'application: ELISA (less than 1 μg/mL). Western blot (1 μg/mL). Immunohistochemistry (cryosections, 1 μg/mL).

Protocole: 1. Stöcker G, Meyer HE, Wagener C and Greiling H (1991): Purification and N-terminal amino acid sequence of a chondroitin sulfate / dermatan sulfate proteoglycan isolated from intima / media preparations of human aorta. Biochem J 274: 415-420. 2. Heintz B, Stöcker G, Rentz U, Melzer H, Mrowka C, Stickeler E, Sieberth HG, Greiling H, Haubeck HD (1995): Decreased glomerular basement membrane heparan sulfate proteoglycan in essential hypertension. Hypertension 25: 399-407. 3. Stefanidis I, Heintz B, Stöcker G, Mrowka C, Sieberth HG, Haubeck HD (1996): Association between heparan sulfate proteoglycan excretion and proteinuria after renal transplantation. J Am Soc Nephrol 7: 1-7. 4. Stöcker G, Stickeler E, Switalla S, Fischer DC, Greiling H, Haubeck HD (1997): Development of an enzyme immuno assay specific for a core protein epitope of a novel small basement membrane associated heparan sulphate proteoglycan from human kidney. Eur J Clin Chem Clin Biochem 35: 95-99.

Restrictions: For Research Use only

Stockage

Buffer: 50 mM TRIS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détail du antigène

Antigène: Small Basement Membrane Heparan Sulfate Proteoglycan

Sujet: The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohisto-chemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.

UniProt: P34741