Actin anticorps

N° du produit ABIN5540756

Cet anticorps Poulet Polyclonal détecte spécifiquement Actin dans WB. Il présente une réactivité envers Lapin.

Aperçu rapide pour Actin anticorps (ABIN5540756)

Antigène: Actin

Reactivité: Lapin

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Actin anticorps

Purification: Affinity Chromatography

Immunogène: Chickens were immunized with actin purified from Rabbit muscle. After epeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 mug/ml of affinity purified anti-Actin IgY, and then filter-sterilized.

Isotype: Ig Fraction

Information d'application

Indications d'application: Quality Control: This anti-Actin preparation was validated by Western blotting on nitrocellulose membranes (at a concentration of 1 μg/mL) using HRP-Conjugated Goat anti-Chicken IgY (1/5000 dilution, Cat.-No AP31795HR-N) as the secondary reagent. Recommended Dilutions: 1/1000-1/2000 for Western blot analysis of Nitrocellulose membranes. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary. Do Not use this product with PVDF, or other nylon-based membranes, because this often produces unacceptably high backgrounds in chemiluminescence studies.

Restrictions: For Research Use only

Stockage

Format: Liquid

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Actin

Antigène: Actin