Doublecortin anticorps

N° du produit ABIN5540930

Cet anticorps Poulet Polyclonal détecte spécifiquement Doublecortin dans IF, ICC et IHC (fro). Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour Doublecortin anticorps (ABIN5540930)

Antigène: Doublecortin (DCX)

Reactivité: Humain, Souris

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp Doublecortin est non-conjugé

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Doublecortin anticorps

Purification: Affinity Chromatography

Immunogène: Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the Doublecortin gene product, but are shared between the Human (CAA06617.1, NCBI) and Mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotype: Ig Fraction

Information d'application

Indications d'application: Immunofluorescence/ Immnunocytochemistry. Immunohistochemistry. Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at a concentration of 2 μg/mL) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody. Recommended Dilutions : 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2 % paraformaldehyde-fixed tissues or cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 2-8°C. This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Doublecortin

Antigène: Doublecortin (DCX)

Autre désignation: doublecortin

UniProt: O43602