NEFH anticorps

N° du produit ABIN5540977

Cet anticorps anti-NEFH est un anticorps Souris Monoclonal détectant NEFH dans WB et IHC (fro). Adapté pour Humain, Rat, Souris, Poulet, Boeuf (Vache), Lapin, Cobaye, Chien, Mouton, Xenopus laevis, Hamster et Poisson zèbre (Danio rerio).

Aperçu rapide pour NEFH anticorps (ABIN5540977)

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivité: Humain, Rat, Souris, Poulet, Boeuf (Vache), Lapin, Cobaye, Chien, Mouton, Xenopus laevis, Hamster, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RNF405

Détail du produit anti-NEFH anticorps

Specificité: RNF405 reacts exclusively with the phosphorylated isoform of the 200 kD Neurofilament protein.

Purification: Purified

Immunogène: Neurofilament preparation of calf brain tissue.

Isotype: IgG2a

Information d'application

Indications d'application: Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin biotinylated Horseradish Peroxidase complex (ABC) as detection reagent, and 1/100-1/500 for Immunoblotting applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Détails sur NEFH

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Autre désignation: neurofilament h (200 kd)

Sujet: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.

UniProt: P12036