COL6 anticorps

N° du produit ABIN5541083

Cet anticorps Lapin Polyclonal détecte spécifiquement COL6 dans IHC (p), IHC (fro), IF, EIA, ICC et RIA. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Poulet et Porc.

Aperçu rapide pour COL6 anticorps (ABIN5541083)

Antigène: COL6 (Collagen, Type VI (COL6))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp COL6 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Radioimmunoassay (RIA)

Détail du produit anti-COL6 anticorps

Specificité: Human Collagen type VI: 100 % binding in RIA at 1/10,000 dilution.

Purification: Affinity Chromatography.

Immunogène: Purified collagen type VI from human placenta.

Information d'application

Indications d'application: ELISA: 1/200 (OD ≥ 500). RIA. Immunofluorescence: 1/80. Immunohistology on Frozen and Paraffin Embedded Sections. For paraffin: 1/400-1/1000, 60 min at RT or 2-8 °C over night for human collagen type VI, lower dilution is recommended for other species). Positive Control: Human placenta. Pretreatment : After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS containing no BSA or preservative.

Stock: 4 °C,-20 °C

Stockage commentaire: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur COL6

Antigène: COL6 (Collagen, Type VI (COL6))

Autre désignation: collagen type vi

Sujet: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea.

UniProt: P12109