Endothelial Cells anticorps (Biotin)

N° du produit ABIN5541247

Cet anticorps anti-Endothelial Cells est un anticorps Rat Monoclonal détectant Endothelial Cells dans IHC (fro). Adapté pour Humain.

Aperçu rapide pour Endothelial Cells anticorps (Biotin) (ABIN5541247)

Antigène: Endothelial Cells

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Endothelial Cells est conjugé à/à la Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1F10

Détail du produit anti-Endothelial Cells anticorps (Biotin)

Specificité: This antibody recognizes an antigen on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not sta in any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent.

Immunogène: Cultured HUVECs

Isotype: IgG2a

Information d'application

Indications d'application: Immunohistochemistry on Frozen sections: 2 μg/mL (1:200). Positive control: Human placenta. Does not react on routinely processed paraffin sections.

Restrictions: For Research Use only

Stockage

Buffer: 0.01 % thimerosal Stabilizers: 10 mg/mL bovine serum albumin

Stock: 4 °C,-20 °C

Stockage commentaire: Store lyophilized at 2-8°C. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Endothelial Cells

Antigène: Endothelial Cells

Sujet: The antigen is found in variable numbers on freshly isolated umbilical vein endotheli al cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40 - 70 % pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercia lly available endothelial cell media apparently have no influence on the antigen expression.