NMDAR2A anticorps (C-Term)

N° du produit ABIN5541473

Cet anticorps Lapin Polyclonal détecte spécifiquement NMDAR2A dans WB et IP. Il présente une réactivité envers Humain, Rat et Souris.

Aperçu rapide pour NMDAR2A anticorps (C-Term) (ABIN5541473)

Antigène: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NMDAR2A est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-NMDAR2A anticorps

Épitope: C-Term

Specificité: Specific for the ~180k NR2A subunit of the NMDA receptor. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.

Purification: Affinity purification using a column to which the fusion protein immunogen was coupled

Immunogène: Fusion protein from the C-terminus of the NR2A subunit of rat NMDA receptor.

Isotype: Ig Fraction

Information d'application

Indications d'application: Western blot: 1/1000. Immunohistochemistry: Immunoprecipitation: 6 μL per 200 μg lysate.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50 % glycerol

Stock: -20 °C

Stockage commentaire: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur NMDAR2A

Antigène: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Autre désignation: nmda receptor 2a

Sujet: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and α -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K /AMPAR). Those that are sensitive to N-methyl-D- aspartate (NMDA) are designated NMDA receptors (N MDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been im plicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002 Wenthold et al., 2003 Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989 Alvestad et al., 2003 Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such cha nnels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

UniProt: Q00959

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity