MESP1 anticorps (AA 1-268)

N° du produit ABIN5541523

Cet anticorps anti-MESP1 est un anticorps Lapin Polyclonal détectant MESP1 dans WB, ICC, IF et IHC (fro). Adapté pour Humain.

Aperçu rapide pour MESP1 anticorps (AA 1-268) (ABIN5541523)

Antigène: MESP1 (Mesoderm Posterior 1 (MESP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MESP1 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-MESP1 anticorps

Épitope: AA 1-268

Specificité: This antibody detetcs Human MesP1. Other species not tested.

Purification: Protein A Chromatography

Immunogène: Highly pure (> 95%) recombinant Human MesP1 (Met1-Lys268) from produced in insect cells (Cat.-No AR31057PU-N).

Information d'application

Indications d'application: Western blot (1-5 μg/mL). Immunofluorescence. Immunohistochemistry on Frozen Sections (1/200).

Restrictions: For Research Use only

Stockage

Buffer: 5 mM PBS, pH 7.2 without preservatives or stabilizers

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur MESP1

Antigène: MESP1 (Mesoderm Posterior 1 (MESP1))

Autre désignation: mesp1

Sujet: ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1 (1,2). As one of the earliest markers, it is expressed specifically in almost all cardiovascular precursors and is required for cardiac morphogenesis (2,3). It was shown that MesP1 is a key factor sufficient to induce the formation of ectopic heart tissue in vertebrates and increase cardiovasculogenesis by ES cells. Electrophysiological analysis showed all subtypes of cardiac ES-cell differentiation (4). MesP1 overexpression and knockdown experiments revealed a prominent function of MesP1 in a gene regulatory cascade, causing Dkk-1-mediated blockade of canonical Wnt-signalling. Independent evidence from ChIP and in vitro DNA-binding studies, expression analysis in wild-type and MesP knockout mice, and reporter assays confirm that Dkk-1 is a direct target of MesP1.

Pathways: Regulation of Muscle Cell Differentiation