ADH1 anticorps (Biotin)
Aperçu rapide pour ADH1 anticorps (Biotin) (ABIN5541710)
Antigène
Voir toutes ADH1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody detects yeast alcohol dehydrogenase. Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-Biotin, anti-rabbit serum as well as purified and partially purified yeast alcohol dehydrogenase.
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Purification
- Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
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Immunogène
- Alcohol dehydrogenase from yeast
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Indications d'application
- Western blot : 1/500-1/5,000. Immunoprecipitation : 1/100. ELISA : 1/5,000-1/20,000. Antibody has been assayed against 1.0 μg of Alcohol dehydrogenase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
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Restrictions
- For Research Use only
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Date de péremption
- 12 months
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- ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))
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Autre désignation
- alcohol dehydrogenase 1
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UniProt
- P00330
Antigène
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