K-RAS anticorps (AA 1-186)

N° du produit ABIN5541964

Cet anticorps anti-K-RAS est un anticorps Souris Monoclonal détectant K-RAS dans WB, IF, FACS, ICC et EIA. Adapté pour Humain.

Aperçu rapide pour K-RAS anticorps (AA 1-186) (ABIN5541964)

Antigène: K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp K-RAS est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Enzyme Immunoassay (EIA)

Clone: AT2F8

Détail du produit anti-K-RAS anticorps

Épitope: AA 1-186

Purification: Protein-A affinity chromatography

Immunogène: Recombinant human KRAS (1-186aa) purified from E. coli.

Information d'application

Indications d'application: The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid. In Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur K-RAS

Antigène: K-RAS (KRAS) (GTPase Kras (KRAS))

Autre désignation: kras

Sujet: KRAS acts as a molecular on/off switch. Once it is turned on, it recruits and activates proteins necessary for the propagation of growth factor and other receptors' sigl such as c-Raf and PI 3-kise. KRAS binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the termil phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, KRAS is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase-activating protein (GAP) class, for example RasGAP. In turn KRAS can bind to proteins of the Guanine Nucleotide Exchange Factor (GEF) class, for example SOS1, which forces the release of bound nucleotide (GDP). Subsequently, KRAS binds GTP present in the cytosol and the GEF is released from ras-GTP.

UniProt: P01116