Transferrin anticorps (N-Term)

N° du produit ABIN5542240

Cet anticorps anti-Transferrin est un anticorps Souris Monoclonal détectant Transferrin dans WB, ICC, IHC (p), IF, EIA, RIA et Func. Adapté pour Humain, Porc et Lapin.

Aperçu rapide pour Transferrin anticorps (N-Term) (ABIN5542240)

Antigène: Transferrin (TF)

Reactivité: Humain, Porc, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Functional Studies (Func)

Clone: HTF-14

Détail du produit anti-Transferrin anticorps

Épitope: N-Term

Specificité: This antibody recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Réactivité croisée (Details): Does not react with Bovine, Sheep, Canine (Dog), Equine (Horse).

Purification: Precipitation methods and ion exchange chromatography (> 95% pure by SDS-PAGE)

Immunogène: Purified porcine transferrin

Isotype: IgG1

Information d'application

Indications d'application: Western blot : non-reducing conditions. Immunohistochemistry on paraffin sections: 10 μg/mL. Positive control: placenta. Immunocytochemistry. ELISA. RIA. Functional application: blocks binding of transferrin to the receptor.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 95 % pure by SDS-PAGE) Buffer System: Phosphate buffered saline (PBS), approx. pH 7.4 0.2 μm filter sterilized

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Transferrin

Antigène: Transferrin (TF)

Autre désignation: transferrin

Sujet: Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 μmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH . Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.

UniProt: P09571

Pathways: Transition Metal Ion Homeostasis