SYN1 anticorps (AA 362-511)

N° du produit ABIN5542390

Cet anticorps anti-SYN1 est un anticorps Souris Monoclonal détectant SYN1 dans WB, ELISA, IHC, ICC et FACS. Adapté pour Humain, Rat, Souris et Singe. Validé indépendamment pour une utilisation dans Multiplex Immunohistochemistry.

Aperçu rapide pour SYN1 anticorps (AA 362-511) (ABIN5542390)

Antigène: SYN1 (Synapsin I (SYN1))

Reactivité: Humain, Rat, Souris, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SYN1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 7H10G6

Détail du produit anti-SYN1 anticorps

Épitope: AA 362-511

Fonction: SYN1 Antibody

Purification: Purified antibody

Immunogène: Purified recombinant fragment of human SYN1 (AA: 362-511) expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104335 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104335

Date: 20.04.2021

Antigène: SYN1

Numéro du lot: 151229

Application validée: Multiplex Immunohistochemistry

Contrôle positif:

FFPE normal human cortex

Contrôle négative:

Unlabeled control (FFPE normal human cortex)

Conclusion:

Passed. The anti-SYN1 antibody ABIN5542390 produces punctate labeling in synapse-rich regions of the human cortex. This pattern is consistent with expected SYN1 localization in synaptic contacts.

Validation Images

FFPE normal human cortex tissue section labeled with anti-SYN1 antibody ABIN5542390 (blue; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and SLC32A1 were labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 500) and anti-SLC32A1 antibody ABIN2855225 (green; bound to fluorophore AF488).

FFPE normal human cortex tissue section labeled with anti-SYN1 antibody ABIN5542390 (red; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and DLG4 were labeled with anti-MAP2 antibody ABIN125739 (cyan; bound to fluorophore ATTO 550) and anti-DLG4 antibody ABIN361694 (green; bound to fluorophore ATTO 550).

Protocole

Anticorps primaire: ABIN5542390

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-SYN1 antibody ABIN5542390 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.

Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Stockage

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Détails sur SYN1

Antigène: SYN1 (Synapsin I (SYN1))

Autre désignation: SYN1

Sujet: This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Poids moléculaire: 74.1 kDa

ID gène: 6853

UniProt: P17600