SYN1 anticorps (AA 362-511)

N° du produit ABIN5542390

Détail du produit anti-SYN1 anticorps

Antigène: SYN1 (Synapsin I (SYN1))

Épitope: AA 362-511

Reactivité: Humain, Souris, Rat, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SYN1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Multiplex Immunohistochemistry (mIHC)

Purification: purified

Immunogène: Purified recombinant fragment of human SYN1 (AA: 362-511) expressed in E. coli.

Clone: 7H10G6

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104335 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104335

Date: 20.04.2021

Antigène: SYN1

Numéro du lot: 151229

Application validée: Multiplex Immunohistochemistry

Contrôle positif:

FFPE normal human cortex

Contrôle négative:

Unlabeled control (FFPE normal human cortex)

Conclusion:

Passed. The anti-SYN1 antibody ABIN5542390 produces punctate labeling in synapse-rich regions of the human cortex. This pattern is consistent with expected SYN1 localization in synaptic contacts.

Validation Images

FFPE normal human cortex tissue section labeled with anti-SYN1 antibody ABIN5542390 (blue; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and SLC32A1 were labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 500) and anti-SLC32A1 antibody ABIN2855225 (green; bound to fluorophore AF488).

FFPE normal human cortex tissue section labeled with anti-SYN1 antibody ABIN5542390 (red; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and DLG4 were labeled with anti-MAP2 antibody ABIN125739 (cyan; bound to fluorophore ATTO 550) and anti-DLG4 antibody ABIN361694 (green; bound to fluorophore ATTO 550).

Protocole

Anticorps primaire: ABIN5542390

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-SYN1 antibody ABIN5542390 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.

Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Stockage

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: 4°C, -20°C for long term storage

Détails sur SYN1

Antigène: SYN1 (Synapsin I (SYN1))

Autre désignation: SYN1 (SYN1 Produits)

Synonymes: anticorps SYN1a, anticorps SYN1b, anticorps SYNI, anticorps Syn-1, anticorps SYN I, anticorps si:dkey-90n12.3, anticorps synapsin I, anticorps synapsin I L homeolog, anticorps SYN1, anticorps Syn1, anticorps syn1.L, anticorps syn1

Sujet:

Description: This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.,

Aliases: SYNI, SYN1a, SYN1b

Poids moléculaire: 74.1 kDa

ID gène: 6853

HGNC: 6853