CRISPR-Cas9 anticorps

N° du produit ABIN5557542

Cet anticorps Souris Monoclonal détecte spécifiquement dans WB. Il présente une réactivité envers Humain.

Aperçu rapide pour CRISPR-Cas9 anticorps (ABIN5557542)

Antigène: CRISPR-Cas9

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Clone: 1E8

Détail du produit

Réactivité croisée: Humain

Purification: Purified by Protein A.

Immunogène: Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (100-200aa)

Isotype: IgM

Information d'application

Indications d'application: WB 1:500-2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CRISPR-Cas9

Sujet:

Synonyms: CRISPR, Cas9, CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, SpCas9, csn1

Background: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

ID gène: 901176

UniProt: Q99ZW2