Recombinant H2AFV anticorps (Sortase,His tag,AVI tag)

N° du produit ABIN5563978

Cet anticorps anti-H2AFV Monoclonal Souris conjugué à Sortase,His tag,AVI tag (ABIN5563978) détecte spécifiquement H2AFV dans WB, ELISA et ChIP. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant H2AFV anticorps (Sortase,His tag,AVI tag) (ABIN5563978)

Antigène: H2AFV (H2A Histone Family, Member V (H2AFV))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp H2AFV est conjugé à/à la Sortase,His tag,AVI tag

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Détail du produit anti-H2AFV anticorps (Sortase,His tag,AVI tag)

Fonction: AbFlex AM-Tag antibody (rAb)

Marque: AbFlex®

Specificité: Drosophila

Attributs du produit: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Purification: Protein A Chromatography

Immunogène: This antibody was raised against Active Motif's unique AM-Tag sequence for use with Tag-ChIP-IT (Cat No. 53022) analysis.

Isotype: IgG2a

Information d'application

Indications d'application: ChIP: 10 μg per ChIP WB: 0.5 - 2 μg/mL dilution Bead-based ELISA: 7 - 560 ng/mL

Commentaires:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur H2AFV

Antigène: H2AFV (H2A Histone Family, Member V (H2AFV))

Autre désignation: Histone H2Av

Sujet: AbFlex AM-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The AM-Tag antibody is specifically designed for use with unique AM-Tag sequence in combination with the Tag-ChIP-IT Kit (Catalog No. 53022). Simply clone your protein of interest into pAM_1C Empty Vector (Catalog No. 53023), or add the AM-Tag sequence to the C-terminus of your protein expression vector. Following transfection, cell lysates are prepared and screened by Western blot for expression of the AM-Tag fusion protein using the AM-Tag antibody. Optimization may be required to identify the optimal conditions for transfection and protein expression. Once transfection conditions have been optimized, the Tag-ChIP-IT Kit can be used to isolate chromatin and perform ChIP. The AM-Tag has minimal cross reactivity with mammalian samples to ensure low background signal. The tag is also unstructured, which allows the tag to protrude from the protein of interest for maximum exposure during immunoprecipitation. This increases the enrichment efficiency of low abundance transcription factors for more reliable and consistent ChIP results. Use of the AM-Tag for chromatin immunoprecipitation avoids the need for protein-specific ChIP-validated antibodies. It also enables the analysis of sequence variants, mutations and truncations on gene regulation.

Poids moléculaire: 15 kDa

NCBI Accession: NP_524519