Intestinal Alkaline Phosphatase anticorps (HRP)

N° du produit ABIN5596690

N° du produit (Fournisseur): 200-6335-0100

Cet anticorps anti-Intestinal Alkaline Phosphatase est un anticorps Mouton Polyclonal détectant Intestinal Alkaline Phosphatase dans WB et DB. Adapté pour Boeuf (Vache).

Aperçu rapide pour Intestinal Alkaline Phosphatase anticorps (HRP) (ABIN5596690)

Antigène: Intestinal Alkaline Phosphatase (ALPI)

Reactivité: Boeuf (Vache)

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Intestinal Alkaline Phosphatase est conjugé à/à la HRP

Application: Western Blotting (WB), Dot Blot (DB)

Détail du produit anti-Intestinal Alkaline Phosphatase anticorps (HRP)

N° du produit (Fournisseur): 200-6335-0100

Fournisseur: Rockland

Fonction: Alkaline Phosphatase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].

Attributs du produit: Synonyms: sheep anti-Alkaline Phosphatase HRP conjugated Antibody, peroxidase conjugated sheep anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Purification: Alkaline phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Alkaline Phosphatase [Calf Intestine]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Alkaline phosphatase peroxidase conjugated has been tested by dot blot and is suitable to be assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:250

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Intestinal Alkaline Phosphatase

Antigène: Intestinal Alkaline Phosphatase (ALPI)

Autre désignation: Calf Intenstinal Alkaline Phosphatase

Sujet: Background: Alkaline phosphatase (ALP, ALKP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65 °C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).

ID gène: 280993

UniProt: P19111