Choline Oxidase anticorps (HRP)

N° du produit ABIN5596705

N° du produit (Fournisseur): 200-103-094-0100

Cet anticorps anti-Choline Oxidase est un anticorps Chèvre Polyclonal détectant Choline Oxidase dans WB, ELISA et IP. Adapté pour Alcaligenes.

Aperçu rapide pour Choline Oxidase anticorps (HRP) (ABIN5596705)

Antigène: Choline Oxidase

Reactivité: Alcaligenes

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Choline Oxidase est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-Choline Oxidase anticorps (HRP)

N° du produit (Fournisseur): 200-103-094-0100

Fournisseur: Rockland

Fonction: Choline Oxidase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species].

Attributs du produit: Synonyms: goat anti-Choline Oxidase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Choline Oxidase Antibody, codA antibody

Purification: Anti-Choline oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Choline Oxidase [Alcaligenes species]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Choline oxidase has been tested by ELISA and western blot and is assayed against 1.0 μg of Choline Oxidase [Alcaligenes species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Choline Oxidase

Antigène: Choline Oxidase

Sujet: Background: Choline oxidase is an enzyme that catalyzes the chemical reaction (choline + O2 <=> betaine aldehyde + H2O2). Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. Choline oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycine, serine, and threonine metabolism.

ID gène: 116367

UniProt: P16101