DLD anticorps (HRP)

N° du produit ABIN5596706

Détail du produit anti-DLD anticorps (HRP)

Antigène: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Reactivité: Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DLD est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Lipoamide Dehydrogenase from other sources is unknown.

Purification: Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].

Immunogène:

Immunogen: Lipoamide Dehydrogenase [Porcine Heart]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Lipoamide Dehydrogenase has been assayed against 1.0 μg of Lipoamide Dehydrogenase [Porcine Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur DLD

Antigène: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Autre désignation: Lipoamide Dehydrogenase (DLD Produits)

Synonymes: anticorps DLDD, anticorps DLDH, anticorps E3, anticorps GCSL, anticorps LAD, anticorps PHE3, anticorps AI315664, anticorps AI746344, anticorps wu:fb24b05, anticorps DLD, anticorps DDBDRAFT_0183800, anticorps DDBDRAFT_0216232, anticorps DDB_0183800, anticorps DDB_0216232, anticorps sc:d0402, anticorps dihydrolipoamide dehydrogenase, anticorps dihydrolipoyl dehydrogenase, anticorps deltaD, anticorps DLD, anticorps Dld, anticorps dldh, anticorps AT4G16155, anticorps CND05840, anticorps bfmBC, anticorps GCSL, anticorps LACBIDRAFT_182385, anticorps UREG_06178, anticorps lpd, anticorps TAGG_RS02070, anticorps Arnit_2606, anticorps Mesil_1945, anticorps Trad_2118, anticorps Acear_0640, anticorps Fbal_0372, anticorps Ilyop_1890, anticorps Ftrac_1733, anticorps Ocepr_1753, anticorps Intca_2017, anticorps Deima_0504, anticorps dld

Sujet:

Synonyms: Dihydrolipoyl dehydrogenase, mitochondrial EC=1.8.1.4 Dihydrolipoamide dehydrogenase Lipoamide Dehydrogenase [Porcine Heart]

Background: Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Gene Name: DLD LAD

ID gène: 397129

UniProt: P09623

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis