AmyA anticorps (HRP)

N° du produit ABIN5596714

N° du produit (Fournisseur): 200-103-234-0100

Cet anticorps Chèvre Polyclonal détecte spécifiquement dans ELISA et WB. Il présente une réactivité envers Rhizopus.

Aperçu rapide pour AmyA anticorps (HRP) (ABIN5596714)

Antigène: AmyA

Reactivité: Rhizopus

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 200-103-234-0100

Fournisseur: Rockland

Fonction: Glucoamylase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus].

Attributs du produit: Synonyms: goat anti-Glucoamylase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glucoamylase Antibody, 1 4 alpha D glucan glucohydrolase antibody, Amyloglucosidase antibody, Gluc 1 antibody, Gluc 2 antibody, Gluc 3 antibody, Glucan 1 4 alpha glucosidase antibody, Glucan 14 alpha glucosidase antibody

Purification: Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Glucoamylase [Rhizopus]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Glucoamylase peroxidase has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Glucoamylase [Rhizopus] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: AmyA

Sujet: Background: Glucoamylase is an amylase that cleaves the last alpha-1,4-glycosidic linkages at the nonreducing end of amylase and amylopectin. It decomposes starch into glucose.

UniProt: P07683