PIPOX anticorps (HRP)

N° du produit ABIN5596798

Détail du produit anti-PIPOX anticorps (HRP)

Antigène: PIPOX (Pipecolic Acid Oxidase (PIPOX))

Reactivité: Microbial

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PIPOX est conjugé à/à la HRP

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Sarcosine Oxidase from other sources is unknown.

Purification: Anti-Sarcosine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial].

Immunogène:

Immunogen: Sarcosine Oxidase [Microbial]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Sarcosine Oxidase has been assayed against 1.0 μg of Sarcosine Oxidase [Microbial] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:2,500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur PIPOX

Antigène: PIPOX (Pipecolic Acid Oxidase (PIPOX))

Autre désignation: Sarcosine Oxidase (PIPOX Produits)

Synonymes: anticorps LPIPOX, anticorps Pso, anticorps SOX, anticorps pipecolic acid and sarcosine oxidase, anticorps pipecolic acid oxidase, anticorps pipecolic acid oxidase L homeolog, anticorps PIPOX, anticorps Pipox, anticorps pipox, anticorps pipox.L

Sujet:

Synonyms: Sarcosine oxidase subunit alpha Sarcosine oxidase subunit EC=1.5.3.1

Background: Sarcosine oxidase is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide, and 5,10-methylenetetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen.

Gene Name: SOXA

ID gène: 10586229

UniProt: Q46337