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Acetylated Lysine anticorps

WB, ELISA, IP, IHC, FACS, FM Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN5596809
  • Antigène Tous les produits Acetylated Lysine
    Acetylated Lysine
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 28
    • 24
    Lapin
    Clonalité
    • 26
    • 25
    Polyclonal
    Conjugué
    • 22
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Cet anticorp Acetylated Lysine est non-conjugé
    Application
    • 49
    • 38
    • 34
    • 23
    • 12
    • 11
    • 11
    • 11
    • 6
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Réactivité croisée (Details)
    The antibody reacts specifically with acetylated lysine residues.
    Purification
    Anti-Lysine Acetylated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.
    Immunogène

    Immunogen: Anti-Lysine Acetylated Antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.

    Immunogen Type: Other

    Isotype
    IgG
  • Indications d'application

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Lysine Acetylated Antibody is suitable for use in ELISA, western blotting, immunofluorescence microscopy, and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, flow cytometry, and immunohistochemistry.

    Western Blot Dilution: 1:500-1:1,000

    Immunoprecipitation Dilution: 10 μg/mg protein sample

    ELISA Dilution: 1:1,000-1:2,500

    IF Microscopy Dilution: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Agent conservateur
    Without preservative
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Zhou, Huang, Zhang, Zhao, Kim, Tu, Zhang, Nowsheen, Zhu, Deng, Chen, Qin, Luo, Liu, Lou, Mutter, Yuan: "The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair." dans: Nature communications, Vol. 11, Issue 1, pp. 2639, (2020) (PubMed).

    Lin, Yuan, Pei, Liu, Ann, Lou: "KAP1 Deacetylation by SIRT1 Promotes Non-Homologous End-Joining Repair." dans: PLoS ONE, Vol. 10, Issue 4, pp. e0123935, (2016) (PubMed).

    Pérez-Luna, Aguasca, Perearnau, Serratosa, Martínez-Balbas, Jesús Pujol, Bachs: "PCAF regulates the stability of the transcriptional regulator and cyclin-dependent kinase inhibitor p27 Kip1." dans: Nucleic acids research, Vol. 40, Issue 14, pp. 6520-33, (2012) (PubMed).

  • Antigène
    Acetylated Lysine
    Autre désignation
    Lysine Acetylated (Acetylated Lysine Produits)
    Synonymes
    anticorps C-K-RAS, anticorps CFC2, anticorps K-RAS2A, anticorps K-RAS2B, anticorps K-RAS4A, anticorps K-RAS4B, anticorps KI-RAS, anticorps KRAS1, anticorps KRAS2, anticorps NS, anticorps NS3, anticorps RASK2, anticorps KRAS proto-oncogene, GTPase, anticorps KRAS
    Classe de substances
    Chemical
    Sujet

    Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

    Background: Acetylation refers to the process of introducing an acetyl group into a compound. Proteins are typically acetylated on lysine residues and this reaction relies on acetyl-coenzyme A as the acetyl group donor. Acetylated Lysine antibody is useful in Cancer, Cell Biology and Chromatin & Nuclear Signaling research.

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