Aspartate Transaminase anticorps (HRP)

N° du produit ABIN5596816

N° du produit (Fournisseur): 200-603-144-0100

Cet anticorps anti-Aspartate Transaminase est un anticorps Mouton Polyclonal détectant Aspartate Transaminase dans ELISA, WB et DB. Adapté pour Porc.

Aperçu rapide pour Aspartate Transaminase anticorps (HRP) (ABIN5596816)

Antigène: Aspartate Transaminase (Ast)

Reactivité: Porc

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Aspartate Transaminase est conjugé à/à la HRP

Application: ELISA, Western Blotting (WB), Dot Blot (DB)

Détail du produit anti-Aspartate Transaminase anticorps (HRP)

N° du produit (Fournisseur): 200-603-144-0100

Fournisseur: Rockland

Fonction: Aspartate Transaminase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].

Attributs du produit: Synonyms: sheep anti-Aspartate Transaminase HRP conjugated Antibody, peroxidase conjugated sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody

Purification: Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Aspartate Aminotransferase (AST) [Pig Heart]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Aspartate Transaminase Peroxidase Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 μg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Aspartate Transaminase

Antigène: Aspartate Transaminase (Ast)

Autre désignation: Aspartate Transaminase

Sujet: Background: Aspartate Transaminase is an important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. It acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Aspartate Transaminase is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells, and it is commonly measured clinically as a marker for liver health.

ID gène: 396967

NCBI Accession: NP_999092

UniProt: P00503