CES3 anticorps (HRP)
Aperçu rapide pour CES3 anticorps (HRP) (ABIN5596851)
Antigène
Voir toutes CES3 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
-
-
N° du produit (Fournisseur)
- 200-4369-0100
-
Fournisseur
- Rockland
-
Fonction
- Esterase Antibody Peroxidase Conjugated
-
Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].
-
Attributs du produit
- Synonyms: rabbit anti-Esterase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase
-
Purification
- Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
-
Immunogène
-
Immunogen: Esterase [Porcine Liver]
Immunogen Type: Native Protein
-
Isotype
- IgG
-
-
-
-
Indications d'application
-
Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Esterase Peroxidase conjugated antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Esterase [Porcine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:250 - 1:1,000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
Concentration
- 1.0 mg/mL
-
Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
Agent conservateur
- Gentamicin sulfate
-
Précaution d'utilisation
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Stock
- 4 °C,-20 °C
-
Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
Date de péremption
- 12 months
-
-
- CES3 (Carboxylesterase 3 (CES3))
-
Autre désignation
- CES3
-
Sujet
- Background: Esterase is a hydrolase enzyme that splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis.
-
ID gène
- 100626873, 343791015
-
UniProt
- Q29550
-
Pathways
- Monocarboxylic Acid Catabolic Process
Antigène
-