CA2 anticorps (HRP)

N° du produit ABIN5596909

Détail du produit anti-CA2 anticorps (HRP)

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Reactivité: Humain, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CA2 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Réactivité croisée (Details): Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.

Purification: Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].

Immunogène:

Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Carbonic anhydrase II has been assayed against 1.0 μg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur CA2

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Autre désignation: Carbonic Anhydrase II (CA2 Produits)

Synonymes: anticorps CG6906, anticorps CT21171, anticorps Dmel\\CG6906, anticorps CA-2, anticorps caII, anticorps car2, anticorps ca ii, anticorps ca-ii, anticorps BETA CA2, anticorps BETA CARBONIC ANHYDRASE 2, anticorps CA18, anticorps CARBONIC ANHYDRASE 18, anticorps CARBONIC ANHYDRASE 2, anticorps T9L3.40, anticorps T9L3_40, anticorps carbonic anhydrase 2, anticorps LOC100135827, anticorps AI131712, anticorps CAII, anticorps Ca2, anticorps Car-2, anticorps Ltw-5, anticorps Lvtw-5, anticorps CA-II, anticorps CAC, anticorps Car2, anticorps CAR3, anticorps Carbonic anhydrase 2, anticorps carbonic anhydrase 2 L homeolog, anticorps carbonic anhydrase 2, anticorps carbonic anhydrase 1, anticorps carbonic anhydrase II, anticorps carbonic anhydrase, alpha type, periplasmic, anticorps CAH2, anticorps ca2.L, anticorps ca2, anticorps CA2, anticorps HCAG_04637, anticorps LOC100135827, anticorps LOC100635510, anticorps Car2, anticorps Ca2, anticorps LOC100154873, anticorps LOC101113341

Sujet:

Synonyms: Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody

Background: Carbonic anhydrase II (CA2), is one of fourteen forms of human α carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

Gene Name: CA2

ID gène: 280740

UniProt: P00921