Laminin anticorps

N° du produit ABIN5596935

Détail du produit anti-Laminin anticorps

Antigène: Laminin (LN)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Laminin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée (Details): Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Anti-Laminin (Human) (RABBIT) Antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.

Purification: Anti-Laminin (RABBIT) Antibody has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Immunogène:

Immunogen: Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50 - 1:200, 5 μg/mL

Application Note: Anti-Laminin (RABBIT) Antibody was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5,000 to 1:10,000. Anti-Laminin (Human) (RABBIT) Antibody was also assayed against 1.0 μg of Laminin in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. Specific conditions should be optimized by researcher.

Western Blot Dilution: 1:500 - 1:,000

ELISA Dilution: 1:4,000 - 1:8,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.04 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Laminin

Antigène: Laminin (LN)

Autre désignation: Laminin (LN Produits)

Synonymes: anticorps LAMC1, anticorps laminin, anticorps laminin, beta 2 (laminin S), anticorps LAMB2

Sujet:

Synonyms: LAMA1 antibody, LAMB1 antibody, Laminin A chain antibody, Laminin alpha 1 antibody, Laminin alpha 1 chain antibody, Laminin B1 chain antibody, Laminin subunit beta 1 antibody

Background: Laminin Antibody is specific to Laminin. Laminins are major proteins in the basal lamina, a protein network foundation for most cells and organs. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. Anti-Laminin antibody is idea for investigators involved in cell cycle protein and extracelluar matrix research.

Gene Name: LAMB1

ID gène: 3912

UniProt: P07942