Pepsin A anticorps (HRP)
Aperçu rapide pour Pepsin A anticorps (HRP) (ABIN5596959)
Antigène
Voir toutes Pepsin A AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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N° du produit (Fournisseur)
- 200-1376-0100
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Fournisseur
- Rockland
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Fonction
- Pepsin Antibody Peroxidase Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach].
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Attributs du produit
- Synonyms: goat anti-Pepsin Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody
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Purification
- Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Immunogène
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Immunogen: Pepsin [Porcine Stomach]
Immunogen Type: Native Protein
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry Dilution: 1:125 - 1:625
Application Note: Anti-Pepsin peroxidase has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided.
Western Blot Dilution: 1:200 - 1:2,500
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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Concentration
- 10.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
Agent conservateur
- Gentamicin sulfate
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Précaution d'utilisation
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- Pepsin A
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Autre désignation
- PGA
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Sujet
- Background: Pepsin is one of three principal protein-degrading, or proteolytic, enzymes in the digestive system, the other two being chymotrypsin and trypsin. Pepsin is commonly used in the preparation produce either an Fab or an F(ab')2 fragment from antibodies.
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ID gène
- 396892
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UniProt
- P00791
Antigène
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