R-Phycoerythrin anticorps (Biotin)
Aperçu rapide pour R-Phycoerythrin anticorps (Biotin) (ABIN5596964)
Antigène
Hôte
Clonalité
Conjugué
Application
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N° du produit (Fournisseur)
- 600-106-387
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Fournisseur
- Rockland
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Fonction
- R-Phycoerythrin Antibody Biotin Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, and R-Phycoerythrin conjugated IgG.
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Attributs du produit
- Synonyms: goat anti R-Phycoerythrin Antibody biotin conjugate, goat anti Phycoerythrin biotin conjugate, goat anti-R-Phycoerythrin biotin conjugation
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Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Immunogène
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Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Immunogen Type: Native Protein
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry Dilution: 1:500 - 1:3,000
Application Note: Anti-R-Phycoerythrin Antibody Biotin Conjugated has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA as well as other immunochemical assays. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular weight fragments is typically noted by western blot.
Western Blot Dilution: 1:2,000 - 1:20,000
ELISA Dilution: 1:200,000
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- R-Phycoerythrin (Phycoerythrin, R-)
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Autre désignation
- R-Phycoerythrin
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Classe de substances
- Chemical
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Sujet
- Background: Anti-R-Phycoerythrin Antibody Biotin Conjugated generated in goat recognizes RPE. Phycoerythrin (PE) is a red protein-pigment present in red algae and cryptophytes and an accessory pigment to the main chlorophyll pigments responsible for photosynthesis. R-phycoerythrin is predominantly produced by red algae. The molecular weight of R-PE is 240 kDa. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-R-PE antibody is conjugated to Biotin.
Antigène
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