R-Phycoerythrin anticorps (Biotin)

N° du produit ABIN5596964

Détail du produit anti-R-Phycoerythrin anticorps (Biotin)

Antigène: R-Phycoerythrin (Phycoerythrin, R-)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp R-Phycoerythrin est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée (Details): This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG.

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:3,000

Application Note: Suitable for immunoblotting (western or dot blot), ELISA as well as other immunochemical assays. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular weight fragments is typically noted by western blot.

Western Blot Dilution: 1:2,000 - 1:20,000

ELISA Dilution: 1:200,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur R-Phycoerythrin

Antigène: R-Phycoerythrin (Phycoerythrin, R-)

Autre désignation: R-Phycoerythrin (R-Phycoerythrin Produits)

Classe de substances: Chemical