Protein G anticorps (Biotin)

N° du produit ABIN5596972

N° du produit (Fournisseur): 200-406-138-0100

Cet anticorps anti-Protein G est un anticorps Lapin Polyclonal détectant Protein G dans ELISA et DB. Adapté pour Streptococcus.

Aperçu rapide pour Protein G anticorps (Biotin) (ABIN5596972)

Antigène: Protein G

Reactivité: Streptococcus

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Protein G est conjugé à/à la Biotin

Application: ELISA, Dot Blot (DB)

Détail du produit anti-Protein G anticorps (Biotin)

N° du produit (Fournisseur): 200-406-138-0100

Fournisseur: Rockland

Fonction: Protein G Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species].

Attributs du produit: Synonyms: rabbit anti-Protein G Antibody biotin conjugation, Rabbit anti Protein G biotin, Rabbit anti PG biotin

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Protein G [Streptococcus species]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Protein G Biotin Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Protein G in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:90,000 to 1:350,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:90,000 - 1:350,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Protein G

Antigène: Protein G

Sujet: Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Protein G antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.