GLN1 anticorps (HRP)

N° du produit ABIN5596985

N° du produit (Fournisseur): 200-103-238-0100

Cet anticorps Chèvre Polyclonal détecte spécifiquement GLN1 dans WB, IP et ELISA. Il présente une réactivité envers Brevibacterium.

Aperçu rapide pour GLN1 anticorps (HRP) (ABIN5596985)

Antigène: GLN1 (Glutamine Synthetase (GLN1))

Reactivité: Brevibacterium

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GLN1 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Détail du produit anti-GLN1 anticorps (HRP)

N° du produit (Fournisseur): 200-103-238-0100

Fournisseur: Rockland

Fonction: Glutamine Synthetase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial].

Attributs du produit: Synonyms: goat anti-Glutamine Synthetase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase

Purification: Glutamine Synthetase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Glutamine Synthetase [native from brevibacterium species]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Glutamine Synthetase has been assayed against 1.0 μg of Glutamine Synthetase [Microbial] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur GLN1

Antigène: GLN1 (Glutamine Synthetase (GLN1))

Autre désignation: Glutamine Synthetase

Sujet: Background: Glutamine Synthetase catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine. Glutamine Synthetase is present predominantly in the brain, kidneys, and liver. In the brain it participates in the metabolic regulation of glutamate, the detoxification of brain ammonia, the assimilation of ammonia, recyclization of neurotransmitters, and termination of neurotransmitter signals. Glutamine Synthetase, in the brain, is found primarily is astrocytes.

ID gène: 3345165

UniProt: Q79VE3

Pathways: Positive Regulation of Peptide Hormone Secretion