Prohibitin 1 / PHB1 (AA 167-261) anticorps

N° du produit ABIN5646917

Cet anticorps anti-Prohibitin 1 / PHB1 Monoclonal Souris (Clone PHB-1882) (ABIN5646917) détecte spécifiquement Prohibitin 1 / PHB1 dans IHC (p) et WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Prohibitin 1 / PHB1 (AA 167-261) anticorps (ABIN5646917)

Antigène: Prohibitin 1 / PHB1

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Classe de qualité: Carrier-free

Clone: PHB-1882

Détail du produit

Épitope: AA 167-261

Fonction: PHB Antibody / Prohibitin (azide and preservative free)

Attributs du produit: This antibody recognizes a protein of 30 kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

Purification: Protein G affinity chromatography

Immunogène: A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the PHB antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the PHB antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the PHB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détail du antigène

Antigène: Prohibitin 1 / PHB1

Sujet: This antibody recognizes a protein of 30 kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

UniProt: P35232