ADO anticorps

N° du produit ABIN5647727

Cet anticorps anti-ADO Polyclonal Lapin (ABIN5647727) détecte spécifiquement ADO dans WB et FACS. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ADO anticorps (ABIN5647727)

Antigène: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ADO est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-ADO anticorps

Purification: Antigen affinity purified

Immunogène: Amino acids E49-E261 from the human protein were used as the immunogen for the ADO antibody.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the ADO antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur ADO

Antigène: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Autre désignation: ADO / 2-Aminoethanethiol dioxygenase

Sujet: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

UniProt: Q96SZ5