KDEL anticorps (N-Term)

N° du produit ABIN5650489

Cet anticorps anti-KDEL est un anticorps Souris Monoclonal détectant KDEL dans WB et IHC. Adapté pour Souris, Rat et Humain.

Aperçu rapide pour KDEL anticorps (N-Term) (ABIN5650489)

Antigène: KDEL (Lys Asp Glu Leu)

Reactivité: Souris, Rat, Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KDEL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 2C1

Détail du produit anti-KDEL anticorps

Épitope: N-Term

Specificité: Detects KDEL tagged proteins.

Purification: Protein G Purified

Immunogène: KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN5650489 was sufficient for detection of KDEL in 10 μg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide

Stock: -20 °C

Détails sur KDEL

Antigène: KDEL (Lys Asp Glu Leu)

Autre désignation: KDEL

Sujet: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.