KDEL anticorps (N-Term) (Biotin)
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- Antigène Tous les produits KDEL (Lys Asp Glu Leu)
- KDEL (Lys Asp Glu Leu)
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Épitope
- N-Term
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Reactivité
- Souris, Rat, Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp KDEL est conjugé à/à la Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificité
- Detects KDEL tagged proteins.
- Purification
- Protein G Purified
- Immunogène
- KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
- Clone
- 2C1
- Isotype
- IgG1
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- Indications d'application
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- WB (1:1000)
- IHC (1:25)
- optimal dilutions for assays should be determined by the user.
- Commentaires
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A 1:1000 dilution of ABIN5650522 was sufficient for detection of KDEL in 10 μg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide
- Stock
- -20 °C
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- Antigène
- KDEL (Lys Asp Glu Leu)
- Autre désignation
- KDEL (Lys Asp Glu Leu Produits)
- Sujet
- The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
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