Nidogen 1 anticorps

N° du produit ABIN5662645

Cet anticorps Rat Monoclonal détecte spécifiquement Nidogen 1 dans WB, IHC, ICC et IHC (fro). Il présente une réactivité envers Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour Nidogen 1 anticorps (ABIN5662645)

Antigène: Nidogen 1 (NID1)

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Nidogen 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ELM1

Détail du produit anti-Nidogen 1 anticorps

Specificité: Mouse.

Purification: Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Immunogène: ELM1 is a rat monoclonal IgG2a antibody derived by fusion of X63 Ag 8.653 mouse myeloma cells with spleen cells from a Fisher rat immunized with a partially purified preparation of laminin from the EHS mouse tumor.

Isotype: IgG2a

Information d'application

Indications d'application: ELM1 reacts with entactin. APPLICATIONS ELM1 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-Nidogen 1 (ABIN5662645)

Couchman, Ljubimov: "Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies." dans: Matrix (Stuttgart, Germany), Vol. 9, Issue 4, pp. 311-21, (1989) (PubMed).

Ljubimov, Afanasjeva, Litvinova, Senin: "Basement membrane components produced by a mouse ascites teratocarcinoma TB24. Analysis with monoclonal and polyclonal antibodies." dans: Experimental cell research, Vol. 165, Issue 2, pp. 530-40, (1986) (PubMed).

Détails sur Nidogen 1

Antigène: Nidogen 1 (NID1)

Autre désignation: Entactin / Nidogen

Sujet: Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or nidogen). Entactin is a sulphated glycoprotein of approximately 150 kD.