Recombinant Fluorescein anticorps (His tag)

N° du produit ABIN5668042

L’anticorps Souris Chimeric anti- a été validé pour in vivo et NC. Il convient pour détecter dans des échantillons de .

Aperçu rapide pour Recombinant Fluorescein anticorps (His tag) (ABIN5668042)

Antigène: Fluorescein

Type d'anticorp: Recombinant Antibody

Fragment: Fab fragment

Hôte: Souris

Clonalité: Chimeric

Conjugué: His tag

Application: In vivo Studies (in vivo), Negative Control (NC)

Clone: 4-4-20 (enhanced)

Détail du produit

Fonction: Anti-Fluorescein [4-4-20 (enhanced)], Mouse Fab fragment, His-Tagged, kappa

Specificité: This antibody is specific for fluorescein I.

Réactivité croisée: Différentes espèces

Attributs du produit:

Original Species of Ab: Human

Original Format of Ab: scFv

Purification: Purified by Immobilized Metal Affinity Chromatography

Immunogène: The original antibody was generated by immunizing BALB/c mice with fluorescein I-keyhole limpet hemocyanin (FlI-KLH).

Isotype: Kappa

Information d'application

Indications d'application: This antibody, a humanized, enhanced scFv of the original mouse IgG2a antibody, was constructed by grafting the CDRs of the original antibody onto the humanized 4D5 anti-HER2 antibody framework, and subsequently enhancing it with respect to folding and stability via randomization in the form of DNA shuffling, as well as oligonucleotides encoding mutations at defined positions (Jung et al., 1999, PMID: 10556036). The original antibody was tested against FlI-BSA. The enhanced antibody fragment was tested against FITC-BSA. This antibody can be used as an isotype control in both in vitro and in vivo applications. This antibody is not conjugated to fluorescein.

Commentaires:

This is a recombinant chimeric Fab fragment created as part of a panel offering antibodies of the same specificity in different formats (species, isotype, subtype and modified versions) for use as isotype controls.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: Fluorescein

Classe de substances: Chemical

Sujet: FL, FlI, FITC, Fluorescein isothiocyanate, fluorescein isothiocyanate, isomer 1, Fluorescyl, Resorcinolphthalein, C.I. 45350, solvent yellow 94, D & C yellow no. 7, Angiofluor, Japan yellow 201, Soap yellow, 3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one