Recombinant Flavivirus Group Antigen anticorps

N° du produit ABIN5668106

Détail du produit

Antigène: Flavivirus Group Antigen

Type d'anticorp: Recombinant Antibody

Reactivité: Dengue Virus, Flavivirus, Virus, West Nile Virus (WNV), Virus de la fièvre jaune

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Specificité: Recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.

Attributs du produit:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Purification: Purified antibody.

Immunogène: Dengue Virus type 2 antigens.

Clone: D1-4G2-4-15 (4G2)

Isotype: IgG2a kappa

Information d'application

Indications d'application: This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis, anti-Yellow Fever Virus or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989). The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004 (PMID: 15564505). Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008). A version of 4G2 directly conjugaed to APC was used by Quicke and co-workers (Quicke, 2016) to detect Zika virus in human placental macrophages by flow-cytometry (Quicke 2016). Ramaiah et al. (2016) used Ab00230-2.0 at a 1:200 dilution to stain for Zika virus in methanol-fixed Vero cells 48 h post-infection.

Restrictions: For Research Use only

Stockage

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Référence

Conzelmann, Zou, Groß, Harms, Röcker, Riedel, Münch, Müller: "Storage-Dependent Generation of Potent Anti-ZIKV Activity in Human Breast Milk." dans: Viruses, Vol. 11, Issue 7, (2020) (PubMed).

Müller, Harms, Krüger, Groß, Joas, Hayn, Dietz, Lippold, von Einem, Schubert, Michel, Mayer, Cortese, Jang, Sandi-Monroy, Deniz, Ebner, Vapalahti, Otto, Bartenschlager, Herbeuval, Schmidt-Chanasit et al.: "Semen inhibits Zika virus infection of cells and tissues from the anogenital region. ..." dans: Nature communications, Vol. 9, Issue 1, pp. 2207, (2018) (PubMed).

Wang, Bardelli, Espinosa, Pedotti, Ng, Bianchi, Simonelli, Lim, Foglierini, Zatta, Jaconi, Beltramello, Cameroni, Fibriansah, Shi, Barca, Pagani, Rubio, Broccoli, Vicenzi, Graham, Pullan, Dowall et al.: "A Human Bi-specific Antibody against Zika Virus with High Therapeutic Potential. ..." dans: Cell, Vol. 171, Issue 1, pp. 229-241.e15, (2017) (PubMed).

Détail du antigène

Antigène: Flavivirus Group Antigen

Classe de substances: Viral Protein

Sujet: Protein E, Envelope Protein