Fibronectin anticorps

N° du produit ABIN5680163

Cet anticorps anti-Fibronectin est un anticorps Lapin Polyclonal détectant Fibronectin dans IF, ELISA, IHC (p) et RIA. Adapté pour Humain.

Aperçu rapide pour Fibronectin anticorps (ABIN5680163)

Antigène: Fibronectin

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Détail du produit anti-Fibronectin anticorps

Specificité: Human fibronectin 100 % , human collagen type I, III, IV and VI <0.1 % (RIA at 1:10000 dilution) Cross-reaction with pig <0.1 %

Réactivité croisée (Details): Human, cross-reaction with pig <0,1 %

Attributs du produit: This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Human fibronectin 100 % , human collagen type I, III, IV and VI <0.1 % (RIA at 1:10000 dilution).

Purification: Affinity purified

Immunogène: Purified fibronectin from human plasma

Isotype: IgG

Information d'application

Indications d'application: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human skin,IFA, IHC(P), ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C.

Concentration: 1 mg/mL

Buffer: Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide!

Agent conservateur: Without preservative

Stock: -20 °C

Détails sur Fibronectin

Antigène: Fibronectin