Fibronectin anticorps

N° du produit ABIN5680165

L’anticorps Lapin Polyclonal anti-Fibronectin a été validé pour WB, IF, ELISA, IHC (p) et RIA. Il convient pour détecter Fibronectin dans des échantillons de Rat.

Aperçu rapide pour Fibronectin anticorps (ABIN5680165)

Antigène: Fibronectin

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Détail du produit anti-Fibronectin anticorps

Specificité: Rat fibronectin 100 % , rat collagen type I, II, III, IV and V <0.1 %, rat elastin <0.1 % (RIA at 1:5000 dilution)

Réactivité croisée (Details): Rat

Attributs du produit: This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Rat fibronectin 100 % , rat collagen type I, II, III, IV and V <0.1 %, rat elastin <0.1 % (RIA at 1:5000 dilution).

Purification: Affinity purified

Immunogène: Purified fibronectin from rat plasma

Isotype: IgG

Information d'application

Indications d'application: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Rat skin,IHC(P), IFA, ELISA, RIA, IB (WB),IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:1000, ELISA ? 1:200 (OD ? 500)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C.

Concentration: 1 mg/mL

Buffer: Lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide!

Agent conservateur: Without preservative

Stock: -20 °C

Détails sur Fibronectin

Antigène: Fibronectin