Collagen Type I anticorps

N° du produit ABIN5680212

Cet anticorps Lapin Polyclonal détecte spécifiquement Collagen Type I dans WB, ELISA, IHC (p), IF et RIA. Il présente une réactivité envers Boeuf (Vache).

Aperçu rapide pour Collagen Type I anticorps (ABIN5680212)

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen Type I est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Détail du produit anti-Collagen Type I anticorps

Specificité: Bovine collagen type I 100 % , bovine collagen II, IX, XI <0.1 %, sheep collagen type I: 50 % (IHC estimated), bovine fibronectin <0.1 %, human collagen type I <2 %, chicken collagen type I <0.1 % (estimates except sheep collagen I from solid phase RIA at dilution 1:500) Cross-reacting with sheep

Réactivité croisée (Details): Cattle, cross-reacting with sheep, swine and human (IHC)

Attributs du produit: Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type I 100 % , bovine collagen II, IX, XI <0.1 %, sheep collagen type I: 50 % (IHC estimated), bovine fibronectin <0.1 %, human collagen type I <2 %, chicken collagen type I <0.1 % (estimates except sheep collagen I from solid phase RIA at dilution 1:500).

Purification: Affinity purified

Immunogène: Purified collagen type I from bovine skin

Isotype: IgG

Information d'application

Indications d'application: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Bovine skin or liver,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:1000, ELISA ? 1:400 (OD ? 500)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Agent conservateur: Without preservative

Stock: -20 °C

Détails sur Collagen Type I

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Autre désignation: Collagen Type I