Cet anticorp Alanine Dehydrogenase est non-conjugé
Application
Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF)
Specificité
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes L-Alanine Dehydrogenase.
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Immunogène
L-Alanine Dehydrogenase isolated and purified from Bacillus subtilis. Freund's complete adjuvant is used in the first step of the immunization procedure.
Reactivité: Bacillus
IP, RIA, WB, DB, EIA, ID, IF
Hôte: Lapin
Polyclonal
Biotin
Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore by adding 1.0 mL of sterile distilled water.
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins.
Agent conservateur
Without preservative
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
This enzyme is a key factor in the assimilation of L-alanine as an energy source through the tricarboxylic acid cycle during sporulation.Synonyms: BSU31930, Stage V sporulation protein N, ald, spoVN