L-Asparaginase 1 anticorps

N° du produit ABIN568115

L’anticorps anti-L-Asparaginase 1 Polyclonal Lapin est utilisé pour la détection de L-Asparaginase 1 dans des échantillons de E. coli. Il a été validé pour WB, IP, EIA, DB, ID, IF et RIA.

Aperçu rapide pour L-Asparaginase 1 anticorps (ABIN568115)

Antigène: L-Asparaginase 1

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunofluorescence (IF), Radioimmunoassay (RIA)

Détail du produit

Specificité: The antibody recognizes L-Asparaginase from Escherichia coli. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Purification: Solid Phase Affinity Chromatography

Immunogène: L-Asparaginase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL of sterile distilled water

Concentration: 2.0 mg/mL

Buffer: PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Détail du antigène

Antigène: L-Asparaginase 1

Sujet: Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L Asparaginase isoenzymes: L Asparaginase I, a low affinity enzyme located in the cytoplasm, and L Asparaginase II, a high affinity secreted enzyme.Synonyms: L-ASNase I, L-asparaginase I, L-asparagine amidohydrolase I, ansA

ID gène: 946278

NCBI Accession: AP_002386

UniProt: P0A962