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CELA1 anticorps

Cet anticorps anti-CELA1 est un anticorps Lapin Polyclonal détectant CELA1 dans WB, IP, EIA, IF, DB, ID et RIA. Adapté pour Porc.
N° du produit ABIN568185

Aperçu rapide pour CELA1 anticorps (ABIN568185)

Antigène

Voir toutes CELA1 Anticorps
CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))

Reactivité

  • 28
  • 8
  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Porc

Hôte

  • 30
  • 5
Lapin

Clonalité

  • 31
  • 4
Polyclonal

Conjugué

  • 22
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp CELA1 est non-conjugé

Application

  • 27
  • 10
  • 9
  • 6
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
  • Specificité

    The antibody recognizes Elastase from Porcine panceas. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

    Purification

    Solid Phase Affinity Chromatography

    Immunogène

    Elastase isolated and purified from Porcine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore by adding 0.5 mL of sterile distilled water

    Concentration

    2.0 mg/mL

    Buffer

    PBS, pH 7.2 stabilized with Dextran, without preservatives and foreign proteins

    Agent conservateur

    Without preservative

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Antigène

    CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))

    Autre désignation

    Elastase-1

    Sujet

    Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5 kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.Synonyms: CELA1, Chymotrypsin-like elastase family member 1, ELA1

    ID gène

    396766

    NCBI Accession

    NP_998988

    UniProt

    P00772
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